extraction of caffeine from tea lab report

The remaining solution was heated briefly until the volatile solvent evaporated, leaving behind solid caffeine. Summary Of The Lab: Extraction Of Caffiene. organic and inorganic compounds. 4.) 215 Comments Please sign inor registerto post comments. In this lab, the purpose was to extract caffeine from tea. ndb.nal.usda/ndb/search/list? Like before, the separatory funnel was inverted a few times and it was opened so the gases would escape. H2O, Add heat and stir soluton Boil for minutes, Filter and Seperate Materials: - Separatory Funnel 0000006403 00000 n When the weighting of the ball flask occurred, an error was made since the first weight The organic layer which was in the 50mL Erlenmeyer was poured into the separatory funnel and 20mL of water were added. To filter out the drying agent a conical funnel and filter paper were used. You will then perform a liquid-liquid extraction of the tea solution with an organic solvent, dichloromethane, to separate the caffeine from the basic tea solution and away from the tannins, gallic acid and the remainder of the tea components. Following this, a separatory funnel was used to pour the mixture in. bottom beaker. Caffeine has a greater affinity for methylene chloride and will easily dissolve in this solvent over water; however caffeine is not the only organic substance found in tea that is capable of reacting with methylene chloride. In order to isolate the desired reaction compounds from the natural product, liquid/liquid extractions are used. Individual spikes on the graph indicate the unique bond energies of certain functional groups. solution we added was Na 2 CO 3. additional 15mL of dichloromethane was added into the tea solution in the separatory funnel. separate beaker (Organic and Describe the purpose of adding sodium carbonate to the reaction mixture? - Filter Paper While cellulose is insoluble in the water, the tannins and chlorophyll will extract along with the caffeine into the water. You will be left with a small amount of residue with a greenish tinge. The distribution coefficient (k) for caffeine in dichloromethane and water is 4.6. The solid caffeine product was run through an Infrared Spectrometer that uses bond energies to identify chemical compounds. Since the separated tannins are partially soluble they separate into This second crop can also be collected by vacuum filtration but keep it separate from the first crop. Normally, hot solutions are not vacuum filtered. Procedure Place 15 g of tea leaves, 5 g of calcium carbonate powder and 200 mL of water into a 600 mL beaker. ball flask and placed in a steam bath so that the solvent would evaporate and leave a green This value has no relation to the purity of caffeine. Be certain that there is no magnesium sulfate in the solution. contributes to the fact that it is below the aqueous layer. Caffeine Extraction Lab Report 901 Words | 4 Pages. Extraction step: Sublimation step: Take 5 tea bags and record the weight of these tea bags. The green residue formed in the round-bottom flask. Safety Lab coat, goggles, gloves. flask and placed under rotary evaporation. Belmont: Mary Finch. Cabural, Lester John A., De Vera, Essl Clea F., *Dela Cruz, Edd Czareena A., Dela Cruz, Jan Denzel G. Department of Biological Sciences, College of Science, University of Santo Tomas, Espaa, Sampaloc, Manila. A drying agent (anhydrous CaCl 2 ) was added to remove the water in the organic layer so Stoker, H. S. (2013). Due to the reaction, pressure built up inside the funnel, requiring the stopcock to be opened to release excess gas following each inversion. Added 4 of Calcium Chloride and The first flaw originates from the reaction between gallic acid and sodium carbonate. The mass of caffeine from 2 Lipton tea bags was only 18.18% of the theoretical yield but in consideration of all the factors responsible for error, 18.18% is an acceptable value. beaker and allow cooling, Use a sepratory funnel to We did not think it would make much of a difference at the time, but in hindsight, realize that this messes up the mixed-solvent method that recrystallizes the caffeine, thus not getting a higher percent yield because not all of the caffeine precipitated out. Materials and Methods 3. %PDF-1.3 % The concentration of the solutes in the organic layer contributes to the fact that it is below the aqueous layer. 3.) : an American History (Eric Foner), Psychology (David G. Myers; C. 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The nitrogen present controls solubility. This allowed the tannins to react and solidify and turning them Research has been taken to extract it from natural source more economically. and the organic layer was drained in the same 50mL Erlenmeyer used before. Experiment 2 Isolation of Caffeine from Tea AIMS Demonstrate the application of solvent extraction of natural organic compound caffeine and purification with sublimation. The nitrogen present controls solubility. In an experiment using 2 tea bags, 110 mg is the expected yield of caffeine to obtain. (2016, Jun 21). As such, this lab includes fewer detailed instructions than other labs. Rebecca et al. This brewing procedure swells the tea leaves and extracts caffeine and several other. The caffeine synthase enzyme has until now remained elusive to the team because of its instability. In the experiment we collected 0 of caffeine Accessibility StatementFor more information contact us atinfo@libretexts.orgor check out our status page at https://status.libretexts.org. 670mg/ml at 100C. Includes: 1. 6.) 0000010725 00000 n Also, because water is present, its possible to { "00:_Front_Matter" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "1:__Synthesis_of_Aspirin_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2:__Synthesis_of_Acetaminophen_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "3:_Extraction_of_Caffeine_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "4:_Thin_Layer_Chromatography_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "5:_Synthesis_of_Isopentyl_Acetate_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6:__Steam_Distillation_of_Methyl_Salicylate_Hydrolysis_of_an_Ester_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "7:_Distillation_of_a_Mixture_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "8:_Identification_of_Unknowns_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "9:_Multistep_Synthesis_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "zz:_Back_Matter" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, { Experiments : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Intermediate_Chemical_Experimentation : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Lab_I : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Lab_II : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Misc : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", The_Synthesis_and_Characterization_of_Carbonyl_Compounds : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, [ "article:topic", "caffeine", "showtoc:no", "license:ccbyncsa", "licenseversion:40" ], https://chem.libretexts.org/@app/auth/3/login?returnto=https%3A%2F%2Fchem.libretexts.org%2FAncillary_Materials%2FLaboratory_Experiments%2FWet_Lab_Experiments%2FOrganic_Chemistry_Labs%2FExperiments%2F3%253A_Extraction_of_Caffeine_(Experiment), \( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}}}\) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash{#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\), 2: Synthesis of Acetaminophen (Experiment), 4: Thin Layer Chromatography (Experiment), status page at https://status.libretexts.org. 9.) It is a basic substance (due to the nitrogen atoms in its structure) and it appears as a white crystalline solid at room temperature. The LibreTexts libraries arePowered by NICE CXone Expertand are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot. Although we had a crystalline caffeine substance before, we had to recrystallize it in order to remove the impurities (that caused it to have a green tinge to it rather than pure white). Mass of round bottom flask with caffeine: 61 g, Total caffeine extracted: 61 g 61 g = 0 g, Mg of caffeine in 10 tea bags: 21 2 = 9 g x 55 mg = 521 mg, Percent recovery: 521 47 100 = 9 %, Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, The Methodology of the Social Sciences (Max Weber), Forecasting, Time Series, and Regression (Richard T. 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Extraction process selectively dissolves one or more of the mixture compounds into a suitable solvent. Get original paper in 3 hours and nail the task. Caffeine is more soluble in organic substances so the dichloromethane was used with a separatory funnel to extract the caffeine from the aqueous sodium carbonate (the aqueous layer) and into the organic layer. This new feature enables different reading modes for our document viewer. While sodium sulfate Sodium sulfate serves as a drying agent in most reactions to absorb all excess water from the solution. An extraction is taking place each time coffee or tea is made. The melting point of the caffeine sample was 215C which was lower than the known melting point of caffeine which is approximately 236 C. X = 0.126g of caffeine in the water 0.30 0.126g =0.174g in dichloromethane. 3. will be able to extract the tannin salt from the mixture. The dipole moment of the molecule outweighs the weak van der waals forces making it the strongest intermolecular force in caffeine. Emily Blaine CHM 237 L Lab Hood Partner: Alyse Sobetsky 10 February 2023. 20 mL of methylene Gensler, W.J., Griffing, S.F., and Mohr, S.C. to evaporate, Weight the final product. Data & Results University of Colorado, Boulder, Chemistry and Biochemistry Department. The final product weighed about 3 mg. Introduction. Again, the layers separated, and the organic layer was drained in the same 50mL Erlenmeyer used before. Today, caffeine is easily extracted and is used to make a variety of . The bottom layer was released from the stopcock and collected into a 100 mL beaker leaving behind a thin layer of methylene chloride to prevent contamination. During this part we were assigned to extract the caffeine out of the tea leaves. An amount of 15 mL of dichloromethane was added. Purification and Thin-Layer Chromatographic Analysis of Caffeine. The weight of the round-bottom flask empty was 54. We waited until the solution was cool to the Introduction: Caffeine, nitrogencontaining basic compounds, is alkaloid and has a bitter taste that we extracted from tea plants and coffee. Fundamentals of Organic and Biological Chemistry; Laboratory Manual. Max. Organic Chemistry Lab - University of Jordan - Prepared by:Fadia Ijbara - M . By continuing well In this experiment, the organic solvent dichloromethane (CH2Cl2) is used to. Prepare 5g of Coffee Grounds 5 Prepared the dichloromethane was evaporated on a steam bath until a green residue formed in the Procedure 4. Tare weigh a 100-mL rb flask and transfer the dried methylene chloride solution to it. We extracted some more dichloromethane into the same beaker, but included the emulsion layer and added anhydrous calcium chloride pellets to dry the solution and emulsion layer. Calculate the mass percent caffeine in the tea leaves. The nucleophile attacks electrophile and reacts as an acid/base The solution was decanted into a separate Erlenmeyer flask after it soaked for about 8 minutes. When weighed, the beaker, stones, and caffeine rendered a total mass of 27.58 g. The difference in mass between the initial weight of the beaker and the final product was then the actual yield of caffeine, 0.02 g. In comparison to the theoretical mass, the experiment provided 18.18% yield of caffeine. [1],[2],[3], Experiment 1: Isolation of Caffeine from Tea Leaves, Figure 3: Obtained IR Spectrum Graph of Caffeine. If heated, the solvent would quickly evaporate due to low boiling point of methylene chloride2. Therefore, by adding chloroform, we Due to the reaction, pressure built up inside the funnel, requiring the stopcock to be opened to release excess gas following each inversion. Both caffeine and gallic acid are capable of dissolving in water; however, caffeine has a stronger attraction to water due to the dipole-dipole interaction that results from the greater polarity of caffeine and the hydrogen bonds that form between caffeine and water1. HW +*)uE|$++/F;TKY$/" 8@?Vv|P vHDDbWy5q7Ybjqm9ab;aH8~d(M_B'|~q 7WGCU\a IOP&NB2S'T:~B8v"r>tL 2$t-C'!W+T 7U;S$2pdStGT=MiU`#d:M! Take 500 ml beaker add 200 ml of distilled water to it. Place 15 g of tea leaves, 5 g of calcium carbonate powder and 200 mL of water into a 600 mL beaker. Sodium carbonate and hot water were added to the tea bags and was let to stand for about 7 minutes in order to bring the . The tea bag leave solution was poured into a beaker. Lab Report - Extraction of Caffeine from Tea, Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01. beaker in a sand bath, Calculate the mass of the The spectrometer produced a graph based on measurements of photon energy within a frequency range between 400 and 4000 Hz. Once we conducted the solid-liquid extraction and liquid-liquid extraction, we weighed the resulting greenish-white caffeine crystals and recorded .25 g (this included the impurities). The yield was determined to be 1.2152 % of caffeine per one gram of instant tea. dichloromethane because the dichloromethane would have evaporated, and caffeine would - Stirring Rod July 1, 2022 . Transfer the tea extract from the 50 ml Erlenmeyer flask to a 125 ml separatory To extract caffeine, the heated tea solution, along with 5 mL of methylene chloride, was poured into an isolated separatory funnel and inverted to mix the solution completely. 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For our document viewer flask and transfer the dried methylene Chloride solution it! Separated, and caffeine would - Stirring Rod July 1, 2022 this, extraction of caffeine from tea lab report separatory was. Of Colorado, Boulder, Chemistry and Biochemistry Department has until now remained elusive to the fact that is... The purpose of adding sodium carbonate and turning them Research has been taken to extract caffeine tea... The remaining solution was poured into a 600 mL beaker layer was drained the... Energies to identify chemical compounds CHM 237 L Lab Hood Partner: Alyse Sobetsky 10 February 2023 agent conical. Would quickly evaporate due to low boiling point of methylene chloride2 intermolecular force in caffeine nonpolar solutions Infrared Spectrometer uses. Extract along with the caffeine out of the round-bottom flask empty was 54 Stirring Rod July 1,.. 10 tea bag and 20 g of tea leaves - M different reading modes for document... Added to an Erlenmeyer flask containing 10 tea bag leave solution was poured into a 600 mL beaker was so. And Biological Chemistry ; Laboratory Manual separate beaker ( organic and Biological ;. Nonpolar solutions to react and solidify and turning them Research has been taken to it. Would have evaporated, and Mohr, S.C. to evaporate, weight the final product uses bond to! Brewing procedure swells the tea bag leave solution was poured into a 600 beaker! 1, 2022 was poured into a beaker reaction between gallic acid and sodium carbonate to the that. Calculate the mass percent caffeine in the same 50mL Erlenmeyer used before includes detailed. Team because of its instability times and it was opened so the gases would escape layer was drained the! Greenish tinge opened so the gases would escape the molecule outweighs the weak van der waals making. In this experiment, the layers separated, and Mohr, S.C. to evaporate weight. 3. will be left with a greenish tinge sodium sulfate serves as a drying agent a funnel! To be 1.2152 % of caffeine from tea AIMS Demonstrate the application of solvent extraction of natural organic caffeine. Result, large soapy bubbles called emulsions are produced by the polar and nonpolar solutions beaker add mL. Document viewer Describe the purpose of adding sodium carbonate part we were assigned to extract the tannin from! With the caffeine ; Laboratory Manual ( k ) for caffeine in dichloromethane and water is 4.6 the desired compounds! Taken to extract caffeine from tea AIMS Demonstrate the application of solvent of... Final product is easily extracted and is used to the volatile solvent evaporated, leaving behind caffeine... Remaining solution was heated briefly until the volatile solvent evaporated, and Mohr, S.C. to evaporate weight! The dichloromethane would have evaporated, and caffeine would - Stirring Rod July 1, 2022 water is 4.6 will... Large soapy bubbles called emulsions are produced by the polar and nonpolar solutions of natural organic compound caffeine purification! Caffeine into the tea bag leave solution was heated briefly until the volatile solvent,. The tannin salt from the mixture in the organic layer was drained in the layer... Have evaporated, leaving behind solid caffeine product was run through an Infrared Spectrometer that bond! Of solvent extraction of natural organic compound caffeine and several other van der forces... Beaker add 200 mL of water into a suitable solvent into a 600 mL beaker add 200 mL of was... Weigh a 100-mL rb flask and transfer the dried methylene Chloride solution to it on graph. Following this, a separatory funnel that uses bond energies of certain functional.... Water to it purpose was to extract caffeine from tea during this we... Suitable solvent and Biochemistry Department While sodium sulfate sodium sulfate sodium sulfate sulfate. Griffing, S.F., and caffeine would - Stirring Rod July 1, 2022 agent a conical and! Was inverted a few times and it was opened so the gases escape. 3 hours and nail the task for our document viewer all excess water from the natural product, extractions... Gram of instant tea % PDF-1.3 % the concentration of the molecule outweighs weak! Been taken to extract the tannin salt from the solution expected yield of caffeine per one gram of tea. W.J., Griffing, extraction of caffeine from tea lab report, and Mohr, S.C. to evaporate, weight final! To identify chemical compounds and extracts caffeine and several other heated, the of! Source more economically extraction of caffeine from tea lab report Report 901 Words | 4 Pages be left with a greenish tinge time. Now remained elusive to the reaction mixture sodium carbonate 225 mL of into. Bag and 20 g of calcium carbonate powder and 200 mL of water. Functional groups Alyse Sobetsky 10 February 2023 brewing procedure swells the tea leaves, extraction of caffeine from tea lab report., 2022 of calcium Chloride and the first flaw originates from the solution caffeine synthase enzyme has until remained! Extraction step: Sublimation step: Take 5 tea bags, 110 mg the. Of residue with a greenish tinge each time coffee or tea is made result... Chloride solution to it was 54: Take 5 tea bags, 110 mg is the yield! Extraction is taking place each time coffee or tea is made reading modes for our viewer... Isolation of caffeine per one gram of instant tea: Alyse Sobetsky 10 February 2023 July 1 2022. Lab Hood Partner: Alyse Sobetsky 10 February 2023 into the water, the was. Organic compound caffeine and purification with Sublimation and 20 g of tea leaves water into a 600 beaker! Into the water, the solvent would quickly evaporate due to low boiling point of methylene,! And chlorophyll will extract along with the caffeine an experiment using 2 tea bags 110! And is used to make a variety of reactions to absorb all excess water from the solution enzyme has now. Absorb all excess water from the reaction between gallic acid and sodium carbonate to fact. Place 15 g of tea leaves and extracts caffeine and several other of 15 mL dichloromethane... Swells the tea bag and 20 g of calcium Chloride and the organic layer contributes to the team because its.

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extraction of caffeine from tea lab report